The PFNT Facility is a coherent set of exploration tools in neurobiology allowing research at the molecular, cellular and integrated levels.
On the 26-31st March, the 14th International Conference on Alzheimer's and Parkinson's Diseases (ADPD) and related neurological disorders was held in Lisbon, Portugal. This year, almost the entire team 1 and team 6 went to this major event and our students: Laurie Arnaud, Laura García González and Dominika Pilat took the opportunity to present their work on a poster.
The LEGO Pumpy (or more officially NanoJ-Fluidics) paper is out in Nature Communications! A joint venture between the INP NeuroCyto team and the Henriques lab, this article (previously available as a preprint on bioRxiv) details how to build a fully open-source multi-channel syringe pumps with LEGO and Arduino.
We have a new preprint out! Want to do good super-resolution images? We have put together all our single molecule localization microscopy (SMLM) tips and tricks. This is a methods paper that describes our SMLM workflow, using benchmark samples such as microtubules and clathrin-coated pits.
Our latest work (previously on bioRxiv) is now published in the Journal of Cell Biology. We collaborated with the Roy lab (UW Madison, USA) and the Jung lab (Ohio University, USA) to reveal a new mechanism of slow axonal transport, based on our previous discovery of actin hotspots and trails.
Christophe was lucky to spend a whole week at the “Microscopie Fonctionnelle en Biologie” aka MiFoBio workshop. Lots of fun attending dozens of cutting-edge workshops, trying super-resolution microscopes, discussing, DJing (!), and presenting the latest work from the lab.
More on Twitter: #MiFoBio2018
The popularization of science is one of the missions of academic research. This article authored by Aurélie Soubéran is intended for children aged 12-13 years and more broadly for the general public, and focuses on a preclinical study on glioblastoma model.
To access it, please download the pdf version.
François Devred has presented his PINT/INP work on non-conventional use of nanoDSF in less than 180 seconds at the 3rd Arbre Mobieu plenary meeting in Zagreb.
See his flash presentation here and the meeting program on Arbre Mobieu website.
This partnership will marry the expertise of Vect-Horus in targeting tumors with its technology VECTrans® and the know-how of RadioMedix in developing and conducting pre-clinical evaluation and clinical trials with radiopharmaceuticals.
For more information, please follow this link or download the pdf version.
As part of her PhD, Sylvie Carmona investigated the beneficial effects of NV669, an aminosterol derived from squalamine on human pancreatic and hepatic cancer models. In vitro results exposed in this paper showed that NV669 inhibited the proliferation of cancer cells, induced cell cycle arrest and subsequent apoptosis. Moreover, NV669 inhibited PTP1B activity and impacted adhesion molecules expression. This suggests that NV669 by inhibiting PTP1B would affect cell contacts and would induce apoptosis.
Les travaux récents de l'équipe NeuroCyto pour dévoiler l'architecture des des axones font l'objet d'un article dans l'édition du vendredi 17 Janvier de La Provence.
Voir l'article de La Provence dans son intégralité ici ou ci-dessous.
Plus d'information dans l'article scientifique publié recemment dans la revue Nature Communications :
L'équipe 8 (GlioME) a le plaisir d'accueillir deux étudiants en Master 2, Anaïs Arathni étudiante en Master Biologie Santé et Emmanuel Snacel-Fazy étudiant en Master Neurosciences. Anaïs travaillera avec Aurélie Tchoghandjian sur la caractérisation des cellules immunitaires recrutées sur le site tumoral suite au traitement par mimétiques de Smac. Emmanuel travaillera avec Chiara Bastiancich sur le microenvironnement de la résection tumorale du glioblastome.
Christophe Leterrier, leader of the NeuroCyto ATIP team, wrote a Spotlight in the Journal of Cell Biology highlighting a nice recent paper from the group of Pei-Lin Cheng in Taiwan. In this article, Lee et al. showed how degradation of the chloride transporter NKCC1 by proteasomes anchored at the AIS have a key role in lowering the intracellular chloride concentration, leading to the perinatal reversal of GABA effect from excitatory to inhibitory.
Thanks to her expertise in the diagnosis of central nervous system tumours arising in children and young adults, Dominique Figarella-Branger from the GlioME team (Team 8) was involved last year in 5 articles published in high-impact factor journals (>18). These articles are becoming references in this field:
- The molecular landscape of ETMR at diagnosis and relapse. Nature. 2019 Dec;576(7786):274-280. PMID: 31802000
Maladie d'Alzheimer : une nouvelle année pleine d'espoir.
In its week-end edition (january the 4th & 5th 2020), the daily newspaper La Marseillaise devoted its "Provence Terre de Science" page to Alzheimer's disease reserach.
On this occasion François Devred (INP team 9 / PINT platform) answered a few questions on the molecular characterization of this complex protein (to be downloaded by clicking here )
First work of 2020 work is out for the NeuroCyto team! A collaboration with Matt Rasband’s lab in Nature Communications. This is a significant paper for the axon initial segment field: Matt’s lab used BioID of key AIS proteins for mapping AIS components. Dozens of new candidates for future studies! We performed super-resolution microscopy of several of the newly identified AIS components. IN particular, we showed that Mical3, a protein linking microtubules and actin, forms clusters along the AIS that are not periodically organized along the actin/spectrin scaffold.
If you speak French, you can listen to “La Méthode Scientifique”, a program on the France Culture radio which highlighted the recent Nature Communications article from the NeuroCyto team.
You can go to the audio clip by following this link and learn more on the France Culture website.