La PFNT (Plateforme NeuroTimone) est un ensemble cohérent d'outils d'exploration en neurobiologie permettant des investigations aux échelles moléculaires, cellulaires et intégrées.
Gaelle Guiraudie Capraz from the NOSE Team met pupils from a CM1 and CM2 class at Jean Jaurès elementary school in Velaux as part of Brain Week.
We hope it will inspire the young one to become future researchers !
Dr. Sarah Moyon, team leader of OligOld (team 4) defended her HDR on Friday, February 23rd, 2024, in front of the jury members: Nicolas Andre (APHM, AMU), Pascale Durbec (IBDM, AMU), Patrice Roll (MMG, AMU), Carlos Parras (ICM, Paris), Maria-Cecilia Angulo (IPNP, Paris) and Anna Williams (University of Edinburgh, UK).
INP team 9 and PINT platform are happy to announce that their work about the Influence of Zinc Ions on the Conformational Stability and Activity of Protein Disulfide Isomerase, authored by Ana Iochabel Soares Moretti, Viktoria E. Baksheeva, Andrei Yu. Roman, Tiphany Coralie De Bessa, François Devred, Hervé Kovacic and Philipp O. Tsvetkov is finally out. A fruitful collaboration between Brazil, Russia and France
You can find its early version by clicking below
Congratulations to Chiara Bordier (SynapTau team) and Thaïs Lestra (Neural Plasticity and Degeneration team), who have been selected by the France Alzheimer's association for their "Parrainage Jeunes Chercheurs" initiative. The objective of this program is to support young researchers throughout their three-year thesis. We are proud of them!
L'AP-HM (Assistance Publique - Hôpitaux de Marseille) a fait le portrait d'Emeline Tabouret pour la 𝙅𝒐𝙪𝒓𝙣𝒆́𝙚 𝙞𝒏𝙩𝒆𝙧𝒏𝙖𝒕𝙞𝒐𝙣𝒂𝙡𝒆 𝒅𝙚𝒔 𝒇𝙚𝒎𝙢𝒆𝙨 𝙙𝒆 𝒔𝙘𝒊𝙚𝒏𝙘𝒆.
Vect-Horus signe un nouvel accord exclusif de licence avec la société Ionis Pharmaceuticals, spécialisée dans le développement de médicaments ciblant les ARN pour le traitement des maladies neurologiques. Vect-Horus, qui est associée à l’INP dans le cadre d’un Laboratoire Commun de Recherche (LabCom) conçoit et développe des vecteurs moléculaires qui permettent une distribution ciblée de molécules thérapeutiques et d’agents d’imagerie dans différents organes et notamment le cerveau.
Aurélie Soubéran and Nathalie Baeza-Kallee represented the GlioME team at the symposium on cortical and spinal organoid at the Institut Jacques Monod in Paris.
The work presented in this poster arose from a collaboration with team 6 (Louise Greetham and Emmanuel Nivet) on the development of an assemboid model (tumor + brain organoid).
The RENOCLIP national meeting was held at the Institut de Cerveau et de la Moelle Epinière in Paris, with the aim of providing an update on research projects and patient management.
Alexandre Bertucci, medical intern and PhD student in the GlioME team, presented the histomolecular profile of young oligodendrogliomas included in the pola network.
Dr. Sarah Moyon, team leader of OligOld (team 4) defended her HDR on Friday, February 23rd, 2024, in front of the jury members: Nicolas Andre (APHM, AMU), Pascale Durbec (IBDM, AMU), Patrice Roll (MMG, AMU), Carlos Parras (ICM, Paris), Maria-Cecilia Angulo (IPNP, Paris) and Anna Williams (University of Edinburgh, UK).
INP team 9 and PINT platform are happy to announce that their work about the Influence of Zinc Ions on the Conformational Stability and Activity of Protein Disulfide Isomerase, authored by Ana Iochabel Soares Moretti, Viktoria E. Baksheeva, Andrei Yu. Roman, Tiphany Coralie De Bessa, François Devred, Hervé Kovacic and Philipp O. Tsvetkov is finally out. A fruitful collaboration between Brazil, Russia and France
Congratulations to Chiara Bordier (SynapTau team) and Thaïs Lestra (Neural Plasticity and Degeneration team), who have been selected by the France Alzheimer's association for their "Parrainage Jeunes Chercheurs" initiative. The objective of this program is to support young researchers throughout their three-year thesis. We are proud of them!
Vect-Horus signe un nouvel accord exclusif de licence avec la société Ionis Pharmaceuticals, spécialisée dans le développement de médicaments ciblant les ARN pour le traitement des maladies neurologiques. Vect-Horus, qui est associée à l’INP dans le cadre d’un Laboratoire Commun de Recherche (LabCom) conçoit et développe des vecteurs moléculaires qui permettent une distribution ciblée de molécules thérapeutiques et d’agents d’imagerie dans différents organes et notamment le cerveau.
Aurélie Soubéran and Nathalie Baeza-Kallee represented the GlioME team at the symposium on cortical and spinal organoid at the Institut Jacques Monod in Paris.
The work presented in this poster arose from a collaboration with team 6 (Louise Greetham and Emmanuel Nivet) on the development of an assemboid model (tumor + brain organoid).
The RENOCLIP national meeting was held at the Institut de Cerveau et de la Moelle Epinière in Paris, with the aim of providing an update on research projects and patient management.
Alexandre Bertucci, medical intern and PhD student in the GlioME team, presented the histomolecular profile of young oligodendrogliomas included in the pola network.
Aurélie Tchoghandjian was a speaker at the Biennal Monegasque de Cancérologie organized by the Centre Scientifique de Monaco and the Fondation Flavien.
To watch Aurélie's presentation, start at 3:06:00 ==> Click here
MT5 MMP- mediated changes in synaptic density, neuronal activity and trafficking of APP b-CTF.
Abstract
Membrane-type 5-matrix metalloproteinase (MT5-MMP) predominantly expressed in neural cells is recently reported to be involved in the proteolytic processing of Amyloid Precursor Protein (APP). The b-C-terminal fragment (b-CTF/C99) generated by cleavage of APP by b-secretase, is the precursor for generation of amyloid beta monomers. The C99 peptide accumulates early in the brains of AD 3xTg and 5xFAD mouse models, even before Aβ and hence plays a major role in the pathology of Alzheimer’s disease. We studied the effect of MT5 domain variants on the levels of C99 as well as its intracellular trafficking in murine primary neuronal cells by over-expressing these via AAV-mediated transduction. The MT5 variants in presence of C99 also impact the synaptic density and neuronal activity suggesting a putative role in the physiology of the neurons.
Francesca Giordano
“Ménage à trois”: role of endoplasmic reticulum-mitochondria contact sites in lipid droplet biogenesis and implication in Alzheimer’s disease
Abstract: Mitochondria (Mito, key for energy production) and lipid droplets (LD, cellular reserve of energy) are essential for cellular homeostasis/bioenergetics. To preserve their morphological and functional identities Mito and LD require a constant and regulated exchange of lipids with the endoplasmic reticulum (ER), major cellular site of lipid synthesis, via membrane contact sites (CS). However, how lipids are shuttled between these organelles and how/where LD originate from the ER is still unclear. Also, dysfunctions of Mito and LD are associated with the pathophysiology of many neurodegenerative disorders such as Alzheimer’s disease (AD). However, how such dysfunctions are established and whether they are functionally connected, remain mysterious. We have recently shown that ER subdomains that contact mitochondria or Mito-Associated Membranes (MAM) are hotspots for LD biogenesis, revealing the existence of a novel tripartite association between Mito, ER and nascent LD. We have found that two lipid transfer proteins ORP5 and ORP8 localize to MAM subdomains where LD originate and that their depletion decreases LD biogenesis in HeLa cells and human hepatocytes. We have also found that ORP5 interacts with seipin, a master regulator of LD biogenesis, and modulates its targeting to MAM-LD contacts. We hypothesize that MAM could play a key role in mediating Mito-LD crosstalk in health and in neurodegeneration. We propose that the early cellular dysfunctions associated to AD involving Mito and LD could be all orchestrated by the MAM. We are currently investigating the function of ORP5/ORP8 and of their binding partners in lipid transfer and storage at MAM-LD contact sites as well as the morphology and remodelling of these contact sites in multiple cell lines including models of AD. Our studies aim to brings novel insights into the metabolic crosstalk between Mito, ER, and LD at membrane contact sites and their implication in neurodegeneration.
EAE models in marmoset for the assessment of cortical demyelination
Maxime Donadieu is a French visiting post-doctoral fellow. After a Bachelor in Biology with a major in Computational Neurosciences (2009-2012, Aix Marseille University), he decided to continue with a Master in Cellular Neurosciences (2013-2015, Aix Marseille University). He was recruited by Siemens S.A.S in 2015, in collaboration with the CRMBM lab (UMR 7339, Aix Marseille University), as an Engineer and also a PhD student. His PhD was focused on the development and the application of MR spectroscopy sequences on healthy subject and MS patients at high (3T) and ultra-high (7T) fields. He defended his PhD in December 2017 and moved in TNS lab in April 2018. The core of his project in TNS is to develop and use translational imaging (conventional, non-conventional MRI and PET scan) but also histology and immunochemistry on EAE marmoset model to understand and quantify the de/remyelination and neurodegenerative processes. The main objective of this project is to develop a remyelinating therapy relevant for MS patient.