Nikon and the Neurophysiopathology Institute at Aix-Marseille University have partnered to create the Nikon Center of Excellence for Neuro-NanoImaging within its cellular imaging facility, focusing on how the latest super-resolutive technique can help in understanding brain cells and their dysfunctions.
The Center uniquely offers three different super-resolution microscopy techniques that are tailored for the subcellular investigation of neuronal function:
• Super-resolved spinning disk (Nikon SoRa) that provides fast, gentle imaging with optical sectioning at ~150 nm resolution, ideal for live-cell imaging.
• Structured Illumination Microscopy (Nikon N-SIM) that allows for robust, multicolor imaging with a 2x gain (~120 nm laterally) compared to diffraction-limited fluorescence microscopy.
• Stochastic Optical Reconstruction Microscopy (Nikon N-STORM) that reaches for ~20 nm detail to dissect the nanoscale organization of macromolecular complexes directly in cells.
The Nikon Center of Excellence at INP brings these cutting-edge techniques to all neurobiology researchers at INP, in Marseille and beyond, with expert advice on how to choose the best technique for a given question and project. Specific development of new sample preparation, imaging and image analysis and processing techniques is performed based on the Center of Excellence microscopes within the facility.
Christophe Leterrier, PhD
Laure Fourel, PhD