Nikon N-SIM

N-SIM Super-Resolution System

The N-SIM provides super-resolution Structured Illumination Microscopy (SIM) on the Ti2-E inverted microscope. It can reach a resolution of 120 nm laterally, 250 nm axially and is compatible with live-cell imaging



* Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

* N-SIM-S illumination module: fast 2D-SIM, 3D-SIM, TIRF-SIM

* LUN-V high power 4-line laser unit (405nm, 488nm, 561nm, 647nm)

* 100X NA 1.49 motorized correction collar objective

* Additional objectives: 40X NA 1.3, 10X

* Hamamatsu Fusion-BT back-thinned sCMOS camera

* Mad City Labs Nano-Z series Z-axis piezo stage

* Tokai Hit stage-top incubation system with CO2 + environmental enclosure for stability

* NIS-Elements software with N-SIM processing and AI modules