Réunion de responsables d'équipe

Thaïs LESTRA 

Modulation of MT5-MMP in Alzheimer's disease and therapeutic modulation using viral-mediated transgenic strategies

Silvia Sposini is a Sir Henry Wellcome postdoctoral fellow at Imperial College London and visiting researcher at the Interdisciplinary Institute for Neurosciences in Bordeaux, France
 

Title :Imaging the dynamics of neuromodulator receptor trafficking

 

Summary:

G protein-coupled receptors (GPCRs) are the largest family of signalling receptors and the main targets of approved drugs. In the nervous system, they bind neuromodulators such as dopamine and noradrenaline to initiate signalling cascades that culminate in the modulation of neuronal functions such as excitability, synaptic transmission and plasticity, and, ultimately, behaviour.

Signalling from neuromodulator GPCRs occurs across extremely accurate spatial and temporal scales. For this, the tight but flexible tuning of GPCR signalling requires highly precise regulatory mechanisms, namely membrane trafficking.

Evidence for the physiological relevance of GPCR trafficking exists in neurons but remains scarce. Moreover, a detailed understanding of how these mechanisms are regulated and which specific cellular function they contribute to, is still missing.

My talk will focus on the information we have gathered so far on neuromodulator receptor trafficking at the molecular scale, with a focus on the tools we have implemented to achieve this. I will also cover our current efforts in understanding how trafficking of GPCRs to intracellular locations shapes neuronal physiology.

 

séminaire équipe 6 - Stem cells, Disease modeling and Neurodegeneration

séminaire équipe 5- SinBiose

Kostas Kostarelos, Head of Nanomedicine Lab, Institut Català de Nanociència i Nanotecnologia, Barcelona (Spain)

Invité de l'équipe Gliome (membre du Jury d'HDR de Chiara Bastiancich)

Cognitive trance: a new tool for well-being, the arts and medicine

What if we had been blind to part of our abilities? What if trance states were part of these abilities? What if trance had therapeutic effects? The trance research institute, which brings together nearly 400 researchers and clinicians, 280 psychologists and 326 other health professionals, has shown that the trance state is one of the non-ordinary states of consciousness in the same way as meditation or hypnosis. This state is accessible to everyone and allows us to connect with our inner selves and the living world. The augmented human being is perhaps not to be found in hyper-technology, but rather in our faculties, which we tend to neglect. During the seminar, Sabrina will present the broad outlines of her thesis work and several participants in her study, members of the laboratory, will talk about their experiences. 

La transe cognitive au service du bien-être, des arts et de la médecine

Et si nous avions été aveugles à une partie de nos capacités ? Et si les états de transe faisaient partie de ces capacités ? Et si la transe avait des effets thérapeutiques ? L’institut de recherche sur la transe, qui regroupe près de 400 chercheurs et cliniciens, 280 psychologues et 326 autres professionnels de santé, a montré que l’état de transe fait partie des états non ordinaires de conscience au même titre que l’état de méditation ou l’hypnose. Cet état est accessible à tous et permet à la fois de se connecter à son moi intérieur et au monde vivant. L’homme augmenté n’est peut-être pas à chercher dans l’hyper-technologie mais plutôt dans nos facultés que nous nous sommes ingéniés à ignorer ou minimiser. A l’occasion de ce séminaire, Sabrina présentera les grandes lignes de son travail de thèse et plusieurs participants de son étude, membres du laboratoire, témoigneront de leurs expériences. 

séminaire équipe 10 Angiogenèse et Microenvironnement Tumoral - Chabane Norhimane - Vaccine Targeting Adrenomedullin System

Understanding synaptic function is essential for investigating brain physiology and pathophysiological changes, such as those occurring in neurodegenerative diseases. Synaptosome isolation, which extracts synaptic terminals containing both pre- and post-synaptic components, provides a valuable tool for studying synaptic biology. Previous research suggests that synaptosome preparation from shock-frozen brain tissue, which is the most common source of human brain synaptosomes, generally yields synaptosomes with a lower respiratory rate, indicating potentially reduced biological functionality compared to fresh tissue. However, detailed comparative studies on the impact of freezing on synaptosome quality are lacking. Our research evaluates how fresh and frozen tissue differ in their suitability for synaptosome isolation, focusing on synaptic protein enrichment, non-synaptic contamination, and the preservation of functionality and structural integrity. We compared synaptosomes isolated from mouse brain tissue that was shock-frozen in liquid nitrogen, slowly frozen with a cryoprotective solution, and freshly processed. Western blot analysis and proteomic profiling were used to assess synaptic protein enrichment and non-synaptic contamination, which will be further validated by electron microscopy. Additionally, functionality assays will determine the impact of tissue state on synaptic exocytosis. Overall, this study aims to evaluate the efficacy of isolating synaptosomes from fresh versus frozen mouse brain tissue. Our findings will contribute to optimizing experimental approaches and improving the comparability of human and preclinical studies in synaptic research.