Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform

authors

• Cao Ruijie
• Li Yaning
• Chen Xin
• Ge Xichuan
• Li Meiqi
• Guan Meiling
• Hou Yiwei
• Fu Yunzhe
• Xu Xinzhu
• Leterrier Christophe
• Jiang Shan
• Gao Baoxiang
• Xi Peng

document type

abstract

Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels. Open-3DSIM also offers the capacity to extract dipole orientation, paving a new avenue for interpreting subcellular structures in six dimensions (xyzθλt). The platform is available as MATLAB code, a Fiji plugin and an Exe application to maximize user-friendliness. Open-3DSIM is a versatile open-source software for high-fidelity reconstruction of three-dimensional structured illumination microscopy data (with polarization). It is available in three convenient forms for user-friendly and customizable applications.

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