Logo FEDER Région Sud

The INteractome Timone Platform (PINT) offers unique expertise in thermodynamic and kinetic analysis of molecular interactions.

PINT is equipped with microcalorimeters (ITC and DSC), nanoDSF, spectrophotometers and an Analytical Ultracentrifuge (UCA), and an optical biosensor based on surface plasmon resonance (SPR).

These techniques all aim to characterize molecular interactions (proteins, nucleic acids, sugars, lipids, small molecules, peptides, proteoliposomes, viral particles, etc...). They have in common the absence of the use of marked reagents, simplicity and speed of implementation. The complementarity of these techniques is widely referenced for the characterization of protein-ligand interactions

This platform is open to the academic and industrial research community.

The PINT platform is located on the 3rd floor of the green wing of the Faculty of Medicine


Microcalorimetry Pole

P.I. : Philipp TSVETKOVFrançois DEVRED

Equipment available:  3 microcalorimeters: ITC200, VP-ITC and VP-DSC allowing both isothermal titration calorimetry (ITC) and temperature scanning calorimetry (DSC) studies to be conducted, as well as the NT.Plex and Tycho nanoDSF.

The ITC makes it possible to characterize intermolecular interactions by obtaining their complete thermodynamic profile: affinity constant, stoichiometry, enthalpy and entropy.

The DSC and the nanoDSF make it possible to determine the thermostability (5-120 °C) of proteins, DNA, micelles... which gives indications on the tertiary structure of molecules and molecular interactions. The nanoDSF through the use of capillaries can also be used for quality control and to screen interactions.

In parallel with our service activity, we are conducting an R&D activity aimed at developing a new diagnostic method by calorimetric profiling of biofluids. In collaboration with INP Team 8 and the Timone Neuro-oncology Department (AP-HM) we are working on the development of a method for monitoring glioblastoma (Tsvetkov et al Oncotarget 2018). In partnership with the QARMA team of the Laboratoire Informatique et Systemes of Aix Marseille University and the company EURANOVA, both specialists in machine learning, we are developing a universal approach aimed at extending this method to other diseases of the nervous system (Alzheimer's, Parkinson's, multiple sclerosis, etc.).


Ultracentrifugation analytique Pole

P.I. : Pascale BARBIER

Equipment available : Beckman XLA Analytical Ultracentrifuge

Analytical UltraCentrifugation (UCA) allows the study of the behaviour of native macromolecules subjected to centrifugal force in solution. With the help of a detection system that measures the macromolecule concentration as a function of the centrifugation radius, we can perform two types of experiments that will lead to complementary informations:

The sedimentation velocity experiment mesures the sedimentation coefficient, the shape, the associative state (monomer, dimer, trimer,...) of a macromolecule. It can also highlight the formation of a complex and allows the determination of its affinity constant.

The sedimentation equilibrium mesures the molecular mass, the associative state (monomer, dimer, trimer,...) and the formation of a macromolecular complex.


Surface Plasmon Resonance pole

P.I. : Géraldine FERRACCI

Equipment available : Biacore T200, Biacore 3000 et ProteOn XPR36

SPR systems are designed to follow in real time interactions between free-labeled biomolecules. One reactant, the ligand, is immobilized on the sensor chip and the others interaction partners, named analytes, are injected at constant flow with a microfluidic circuit other the interface. The SPR principle is to follow mass changes on the surface of the sensor chip, induced by molecular complex formation and dissociation.

The use of real-time measurement allows to provide association (ka) and dissociation (kd) rate constant of the interaction and to deduce its affinity (KD). With an automated, parallelized and miniaturized mode of analysis, SPR optical biosensors also allow to define thermodynamic constants, the active concentration of an analyte, epitope mapping or study molecules in complex environments and perform micropurification and recovery of unknown partners.


Proteomic analysis CAPM pole

P.I. : Claude Villard,

Equipment available : Micro-HPLC Smart System BioRad ; Ultraflex II TOF-TOF Bruker ; Q-TOF Ultima Waters ; TECAN EVO 100 ; Nano UHPLC DIONEX 3000.

Mass spectrometry is a physical analytical technique that allows molecules to be detected and identified by measuring their mass and characterizing their chemical composition.

- Whole mass

- PMF or MSMS identification

- iTRAQ Quantification

- Sequencing de novo




Non exhaustive list of PINT’s collaborators :

Marseille medical genetics, Inserm UMR U1251, Marseille (Marc Bartoli) - Institut de Chimie de Nice, UMR CNRS 7272, Nice (Rachid Benhida) - Centre européen de recherche en imagerie médicale, Marseille (Benjamin Guillet) - INP, Institut de NeuroPhysiopathologie, UMR CNRS 7051, Marseille (Francois Devred, Emeline Tabouret) - CRCM, Centre de recherche en cancérologie de Marseille, Inserm U1068, Marseille (Joseph Ciccolini, Eddy Pasquier) - Laboratoire d'Ingénierie des Systèmes Macromoléculaires,UMR CNRS 7255, Marseille (James Sturgis) - Grenoble Institut Neurosciences, Inserm UMR U1216, Grenoble (Isabelle Arnal) - Architecture et Fonction des Macromolécules Biologiques, CNRS UMR 7257, Marseille, (Sonia Longhi) -  Institut de Microbiologie de la Méditerranée, Laboratoire de chimie Bactérienne, CNRS UMR 7283, Marseille, (Romé Voulhoux) - Institut de Microbiologie de la Méditerranée, Bioénergétique et Ingénierie des Protéines, CNRS UMR 7281, Marseille, (Brigitte Gontéro)



PFNT is a facility labeled AMU.


Image icon logo ARC sans texte15.07 KB
Image icon logo Gefluc137.86 KB
Image icon Logo Aviesan ITMO Cancer7.39 KB
Image icon logo ARTC Sud32.83 KB
Image icon logo Region Sud84.93 KB
Image icon logo canceropole paca36.03 KB


  1. MEDIM workshop 3rd edition

    INP platform PINT together with AFMB and IMM are glad to announce their third inter-platform interactome workshop entitled "Methods to study Molecular Interactions" MEDIM 2024, that will take place from monday April 15th to friday 19th 2024

    Looking to confirm the interaction between a drug and its target?
    Wanting to characterize the interaction between a receptor and its ligand?

  2. Journée de la Recherche de la Faculté de Pharmacie 2023

    Comme chaque année la Faculté de Pharmacie de Marseille met à l'honneur ses doctorants, chercheurs et enseignants-chercheurs lors de sa Journée de la Recherche qui avait lieu cette année le 12 octobre 2023;  Au cours de cette journée les enseignants chercheurs de la faculté Pascale Barbier, François Devred, Hervé Kovacic (équipe 9), et Alessandra Pagano (équipe 8) accompagnés de  Chiara Bastiancich, Aurélie Souberan (équipe 8) et Maud Gratuze (équipe 2') étaient présents pour répondre aux nombreuses questions des étudiants de Pharmacie et/ou de Master concernant la recher

  3. l'INP à la Nuit Européenne des Chercheurs 2023

    Chaque année, la Nuit européenne des chercheur·e·s crée la rencontre entre un millier de publics et une centaine de scientifiques d’Aix-Marseille Université. À travers des formats de rencontre originaux tels que des tête-à-tête, un escape game, des expériences scientifiques, un bureau de désinformation, un cinéma… idées et connaissances se partagent dans un Dock des suds transformé avec la complicité de scénographes marseillaises. Cette année l'NP y tenait un stand autour de la thématique Vieillissement et maladie d'Alzheimer.

  4. INP joins "Net4Brain: A comprehensive Network Against Brain Cancer" COST Action

    INP Team 9 is pleased to announce the launch of the COST Action "Net4Brain: A comprehensive Network Against Brain Cancer". This initiative, led by Prof. Xinzhong Li (Teesside University), brings together laboratories and universities from over 20 countries (Belgium , Bosnia and Herzegovina , Czech Republic , Estonia , France , Germany , Italy , Latvia , Luxembourg , Malta , Netherlands , Poland , Portugal , Romania , Serbia , Slovenia , Spain , Sweden , Switzerland , Turkey , United Kingdom, Israel, China, United States) including AMU / INP through the participation of François Devred.

  5. Financement Canceropole : Repositionnement de molécules en prématuration
  6. BSI2023 : 3rd Integrative Structural Biology meeting in Marseille

    INP, more precisely PINT platform, along with 13 laboratories and institutes affiliated with AMU (*) is co-organizing the 3rd Integrative Structural Biology meeting (BSI 2023) with the Association Française de Cristallographie (AFC) and the Société Française de Biophysique (SFB). The meeting will take place in Marseille in the Faculty of Medical Sciences on the Timone Campus on November 13-17, 2023.

    Save the date and follow us on Twitter (@BSI2023) for updated information!

  7. INP Team 8 and 9 joint publication : Plasma nanoDSF Denaturation Profile at Baseline Is Predictive of Glioblastoma EGFR Status

    INP, Molecular Interaction & Pharmacology (team 9) and GlioME: Gliomagenesis and MicroEnvironment (team 8) are glad to share the release of their new work "Plasma nanoDSF Denaturation Profile at Baseline Is Predictive of Glioblastoma EGFR Status" which was done in collaboration with data scientists from Laboratoire Hubert Curien, UJM-Saint-Etienne and Laborat

  8. INP & Vect-Horus running against Alzheimer's disease

    On december 10th 2022, PINT INP and Vect-Horus members met in the Hippodrome Borély for the "Course des Lumières" organized by Foundation Recherche Alzheimer. It was freezing but together with 600 or something others, they walked 5 km or ran 8 km, to light up the night against Alzheimer's disease and for all patients, carrying a luminous object. See a few pictures below 


Job offers

Poste pourvu : Platform engineer : Conformational stability of proteins and protein ligands interactions

The Platform Interactome Timone of the Institute of Neurophysiopathology (INP) located at the Medical Faculty of AMU at Timone campus (Marseille) is seeking a dynamic and talented engineer with strong background in biophysical methods and protein biochemistry for participation in several interdisciplinary projects. Our unit uses biophysical techniques (Isothermal titration calorimetry, differential scanning calorimetry, differential scanning fluorimetry, dynamic light scattering, surface plasmon resonance etc.) to study the molecular mechanisms implicated in neurodegeneration and to develop new diagnostic methods.

The engineer will be involved in developing a novel universal diagnostic method based on denaturation profiles of human plasma samples that will be analyzed with the help of machine learning algorithms. Also, he/she will participate in the screening of a large library of inhibitors of protein-protein interactions to find new compounds that impact microtubular cytoskeleton.

The ideal candidate must have skills in biophysical methods, molecular biology, and biochemistry.
The position is opened for one year, renewable up to 2 years starting  from september 2021. Net wage : 1500-1800 net
Address : Plateforme PINT, Institut de NeuroPhsyiopathology, UMR 7051, Faculté des Sciences Médicales et Paramédicales, 27 Bd Jean Moulin, 13005 Marseille
website :

Applicants must email a CV, a statement of interest and contact details for 2-3 references to

Relevant publications:

2020     An AI-powered blood test to detect cancer using nanoDSF

2020     In silico molecular target prediction unveils mebendazole as a potent MAPK14 inhibitor

2019     Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC)

2018     Differential scanning calorimetry of plasma in glioblastoma: toward a new prognostic/monitoring tool