LDLR-mediated targeting and productive uptake of siRNA-peptide ligand conjugates in vitro and in vivo

authors

• B Broc
• K Varini
• R Sonnette
• B Pecqueux
• F Benoist
• M Thomas
• M Masse
• Y Mechioukhi
• G Ferracci
• M David
• J Temsamani
• M Khrestchatisky
• G Jacquot
• P Lécorché

abstract

siRNAs have become one of the most promising therapeutic agents because of their specificity and their potential to modulate the expression of gene-related diseases. Any gene of interest can be potentially up or down-regulated, making RNA-based technology the healthcare breakthrough of our era. However, the functional and specific delivery of siRNAs into tissues of interest and into the cytosol of target cells remains highly challenging, mainly due to the lack of efficient and selective delivery systems. Among the variety of carriers for siRNA delivery, peptides have become essential candidates because of their high selectivity, stability and conjugation versatility. Here, we describe the development of molecules encompassing siRNAs against SOD1 , conjugated to peptides that target the LDLR, and their biological evaluation both in vitro and in vivo . Abstract Figure GRAPHICAL ABSTRACT

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