Circadian RNA expression elicited by 3’-UTR IRAlu-paraspeckle associated elements

authors

• Torres Manon
• Becquet Denis
• Blanchard Marie-Pierre
• Guillen Séverine
• Boyer Bénédicte
• Moreno Mathias
• Franc Jean-Louis
• François-Bellan Anne-Marie

keywords

• Cell Biology
• Genes and Chromosomes
• Paraspeckles

document type

abstract

Circadian clocks regulate rhythmic gene expression levels by means of mRNA oscillations that are mainly driven by post-transcriptional regulation. We identified a new post-transcriptional mechanism, which involves nuclear bodies called paraspeckles. Major components of paraspeckles including the long noncoding RNA Neat1, which is the structural component, and its major protein partners, as well as the number of paraspeckles, follow a circadian pattern in pituitary cells. Paraspeckles are known to retain within the nucleus RNAs containing inverted repeats of Alu sequences. We showed that a reporter gene in which these RNA duplex elements were inserted in the 3’-UTR region displayed a circadian expression. Moreover, circadian endogenous mRNA associated with paraspeckles lost their circadian pattern when paraspeckles were disrupted. This work not only highlights a new paraspeckle-based post-transcriptional mechanism involved in circadian gene expression but also provides the list of all mRNA associated with paraspeckles in the nucleus of pituitary cells

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