Automating multimodal microscopy with NanoJ-Fluidics

authors

• Almada Pedro
• Pereira Pedro M.
• Culley Siân
• Caillol Ghislaine
• Boroni-Rueda Fanny
• Dix Christina L
• Baum Buzz
• Laine Romain F
• Leterrier Christophe
• Henriques Ricardo

abstract

Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.

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