First chemical synthesis of a scorpion α-toxin affecting sodium channels: The Aah I toxin of Androctonus australis hector

authors

  • M'Barek Sarrah
  • Fajloun Ziad
  • Cestèle Sandrine
  • Devaux Christiane
  • Mansuelle Pascal
  • Mosbah Amor
  • Jouirou Besma
  • Mantegazza Massimo
  • van Rietschoten Jurphaas
  • El Ayeb Mohamed
  • Rochat Hervé
  • Sabatier Jean-Marc
  • Sampieri François

keywords

  • Oxidation/refolding
  • Scorpion α -toxin
  • Aah I
  • Solid-phase peptide synthesi s
  • Sodium channel

abstract

Aah I is a 63-residue alpha-toxin isolated from the venom of the Buthidae scorpion Androctonus australis hector, which is considered to be the most dangerous species. We report here the first chemical synthesis of Aah I by the solid-phase method, using a Fmoc strategy. The synthetic toxin I (sAah I) was renatured in DMSO-Tris buffer, purified and subjected to thorough analysis and comparison with the natural toxin. The sAah I showed physico-chemical (CD spectrum, molecular mass, HPLC elution), biochemical (amino-acid composition, sequence), immunochemical and pharmacological properties similar to those of the natural toxin. The synthetic toxin was recognized by a conformation-dependent monoclonal anti-Aah I antibody, with an IC50 value close to that for the natural toxin. Following intracerebroventricular injection, the synthetic and the natural toxins were similarly lethal to mice. In voltage-clamp experiments, Na(v) 1.2 sodium channel inactivation was inhibited by the application of sAah I or of the natural toxin in a similar way. This work describes a simple protocol for the chemical synthesis of a scorpion alpha-toxin, making it possible to produce structural analogues in time.

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