NeuroTimone Facility (PFNT)

The PFNT Facility is a coherent set of exploration tools in neurobiology allowing research at the molecular, cellular and integrated levels.

News

  1. Brain week : NOSE team

    Gaelle Guiraudie Capraz from the NOSE Team met pupils from a CM1 and CM2 class at Jean Jaurès elementary school in Velaux as part of Brain Week.

    We hope it will inspire the young one to become future researchers !

  2. First Climate Fresk workshop at the INP !

    On February 8, 2024, 8 INP and 2 NeuroMarseille staff took part in a Climate Fresk workshop, led by our Sustainable Development / Ecoresponsibility referent Louise Greetham, freshly trained by the CNRS to lead these environmental transition workshops. During 3 hours, participants were able to understand the causes of climate change and the issues involved. 
    They also brainstormed solutions to be implemented within our institute in order to reduce our environmental impact. 

     

     

     

  3. HDR defense - Team 4 OligOld

    Dr. Sarah Moyon, team leader of OligOld (team 4) defended her HDR on Friday, February 23rd, 2024, in front of the jury members: Nicolas Andre (APHM, AMU), Pascale Durbec (IBDM, AMU), Patrice Roll (MMG, AMU), Carlos Parras (ICM, Paris), Maria-Cecilia Angulo (IPNP, Paris) and Anna Williams (University of Edinburgh, UK).

  4. Team 9 and PINT new publication is out

    INP team 9 and PINT platform are happy to announce that their work about the Influence of Zinc Ions on the Conformational Stability and Activity of Protein Disulfide Isomerase, authored by Ana Iochabel Soares Moretti, Viktoria E. Baksheeva, Andrei Yu. Roman, Tiphany Coralie De Bessa, François Devred, Hervé Kovacic and Philipp O. Tsvetkov is finally out. A fruitful collaboration between Brazil, Russia and France 

    You can find its early version by clicking below  

  5. Chiara et Thaïs sélectionnées à l'action Parrainage Jeunes Chercheurs de l'association France Alzheimer

    Congratulations to Chiara Bordier (SynapTau team) and Thaïs Lestra (Neural Plasticity and Degeneration team), who have been selected by the France Alzheimer's association for their "Parrainage Jeunes Chercheurs" initiative. The objective of this program is to support young researchers throughout their three-year thesis. We are proud of them!

  6. [ 𝗗𝗶𝗺𝗮𝗻𝗰𝗵𝗲 𝟭𝟭 𝗳𝗲́𝘃𝗿𝗶𝗲𝗿 ] 𝙅𝒐𝙪𝒓𝙣𝒆́𝙚 𝙞𝒏𝙩𝒆𝙧𝒏𝙖𝒕𝙞𝒐𝙣𝒂𝙡𝒆 𝒅𝙚𝒔 𝒇𝙚𝒎𝙢𝒆𝙨 𝙙𝒆 𝒔𝙘𝒊𝙚𝒏𝙘𝒆

    L'AP-HM (Assistance Publique - Hôpitaux de Marseille) a fait le portrait d'Emeline Tabouret pour la 𝙅𝒐𝙪𝒓𝙣𝒆́𝙚 𝙞𝒏𝙩𝒆𝙧𝒏𝙖𝒕𝙞𝒐𝙣𝒂𝙡𝒆 𝒅𝙚𝒔 𝒇𝙚𝒎𝙢𝒆𝙨 𝙙𝒆 𝒔𝙘𝒊𝙚𝒏𝙘𝒆.

  7. Vect-Horus conclut un accord de licence exclusif avec Ionis Pharmaceuticals

    Vect-Horus signe un nouvel accord exclusif de licence avec la société Ionis Pharmaceuticals, spécialisée dans le développement de médicaments ciblant les ARN pour le traitement des maladies neurologiques. Vect-Horus, qui est associée à l’INP dans le cadre d’un Laboratoire Commun de Recherche (LabCom) conçoit et développe des vecteurs moléculaires qui permettent une distribution ciblée de molécules thérapeutiques et d’agents d’imagerie dans différents organes et notamment le cerveau. 

  8. Poster at the Symposium on cortical and spinal organoids

    Aurélie Soubéran and Nathalie Baeza-Kallee represented the GlioME team at the symposium on cortical and spinal organoid at the Institut Jacques Monod in Paris. 

     

    The work presented in this poster arose from a collaboration with team 6 (Louise Greetham and Emmanuel Nivet) on the development of an assemboid model (tumor + brain organoid). 

Pages

INP in numbers

  • 126 members
  • 44 researchers
  • 48 research assistants
  • 12 post-docs
  • 11 PhD

 

First Climate Fresk workshop at the INP !

On February 8, 2024, 8 INP and 2 NeuroMarseille staff took part in a Climate Fresk workshop, led by our Sustainable Development / Ecoresponsibility referent Louise Greetham, freshly trained by the CNRS to lead these environmental transition workshops. During 3 hours, participants were able to understand the causes of climate change and the issues involved. 
They also brainstormed solutions to be implemented within our institute in order to reduce our environmental impact. 

 

 

 

English

HDR defense - Team 4 OligOld

Dr. Sarah Moyon, team leader of OligOld (team 4) defended her HDR on Friday, February 23rd, 2024, in front of the jury members: Nicolas Andre (APHM, AMU), Pascale Durbec (IBDM, AMU), Patrice Roll (MMG, AMU), Carlos Parras (ICM, Paris), Maria-Cecilia Angulo (IPNP, Paris) and Anna Williams (University of Edinburgh, UK).

Undefined

Team 9 and PINT new publication is out

INP team 9 and PINT platform are happy to announce that their work about the Influence of Zinc Ions on the Conformational Stability and Activity of Protein Disulfide Isomerase, authored by Ana Iochabel Soares Moretti, Viktoria E. Baksheeva, Andrei Yu. Roman, Tiphany Coralie De Bessa, François Devred, Hervé Kovacic and Philipp O. Tsvetkov is finally out. A fruitful collaboration between Brazil, Russia and France 

Undefined

Chiara et Thaïs sélectionnées à l'action Parrainage Jeunes Chercheurs de l'association France Alzheimer

Congratulations to Chiara Bordier (SynapTau team) and Thaïs Lestra (Neural Plasticity and Degeneration team), who have been selected by the France Alzheimer's association for their "Parrainage Jeunes Chercheurs" initiative. The objective of this program is to support young researchers throughout their three-year thesis. We are proud of them!

Undefined

[ 𝗗𝗶𝗺𝗮𝗻𝗰𝗵𝗲 𝟭𝟭 𝗳𝗲́𝘃𝗿𝗶𝗲𝗿 ] 𝙅𝒐𝙪𝒓𝙣𝒆́𝙚 𝙞𝒏𝙩𝒆𝙧𝒏𝙖𝒕𝙞𝒐𝙣𝒂𝙡𝒆 𝒅𝙚𝒔 𝒇𝙚𝒎𝙢𝒆𝙨 𝙙𝒆 𝒔𝙘𝒊𝙚𝒏𝙘𝒆

L'AP-HM (Assistance Publique - Hôpitaux de Marseille) a fait le portrait d'Emeline Tabouret pour la 𝙅𝒐𝙪𝒓𝙣𝒆́𝙚 𝙞𝒏𝙩𝒆𝙧𝒏𝙖𝒕𝙞𝒐𝙣𝒂𝙡𝒆 𝒅𝙚𝒔 𝒇𝙚𝒎𝙢𝒆𝙨 𝙙𝒆 𝒔𝙘𝒊𝙚𝒏𝙘𝒆.

Undefined

Vect-Horus conclut un accord de licence exclusif avec Ionis Pharmaceuticals

Vect-Horus signe un nouvel accord exclusif de licence avec la société Ionis Pharmaceuticals, spécialisée dans le développement de médicaments ciblant les ARN pour le traitement des maladies neurologiques. Vect-Horus, qui est associée à l’INP dans le cadre d’un Laboratoire Commun de Recherche (LabCom) conçoit et développe des vecteurs moléculaires qui permettent une distribution ciblée de molécules thérapeutiques et d’agents d’imagerie dans différents organes et notamment le cerveau. 

Undefined

Poster at the Symposium on cortical and spinal organoids

Aurélie Soubéran and Nathalie Baeza-Kallee represented the GlioME team at the symposium on cortical and spinal organoid at the Institut Jacques Monod in Paris. 

 

The work presented in this poster arose from a collaboration with team 6 (Louise Greetham and Emmanuel Nivet) on the development of an assemboid model (tumor + brain organoid). 

Undefined

Next seminars

22 Mar 2024 14:30

Team 1: Shweta SONAWANE

Salle 11 (1er étage Aile rouge)

MT5 MMP- mediated changes in synaptic density, neuronal activity and trafficking of APP b-CTF.

Abstract

Membrane-type 5-matrix metalloproteinase (MT5-MMP) predominantly expressed in neural cells is recently reported to be involved in the proteolytic processing of Amyloid Precursor Protein (APP). The b-C-terminal fragment (b-CTF/C99) generated by cleavage of APP by b-secretase, is the precursor for generation of amyloid beta monomers. The C99 peptide accumulates early in the brains of AD 3xTg and 5xFAD mouse models, even before Aβ and hence plays a major role in the pathology of Alzheimer’s disease. We studied the effect of MT5 domain variants on the levels of C99 as well as its intracellular trafficking in murine primary neuronal cells by over-expressing these via AAV-mediated transduction. The MT5 variants in presence of C99 also impact the synaptic density and neuronal activity suggesting a putative role in the physiology of the neurons.

29 Mar 2024 11:00

Team 6: Guest Seminar: Francesca Giordano

Amphi 6 (5e aile verte - 5th green section)

Francesca Giordano

“Ménage à trois”: role of endoplasmic reticulum-mitochondria contact sites in lipid droplet biogenesis and implication in Alzheimer’s disease

Abstract:  Mitochondria (Mito, key for energy production) and lipid droplets (LD, cellular reserve of energy) are essential for cellular homeostasis/bioenergetics. To preserve their morphological and functional identities Mito and LD require a constant and regulated exchange of lipids with the endoplasmic reticulum (ER), major cellular site of lipid synthesis, via membrane contact sites (CS). However, how lipids are shuttled between these organelles and how/where LD originate from the ER is still unclear. Also, dysfunctions of Mito and LD are associated with the pathophysiology of many neurodegenerative disorders such as Alzheimer’s disease (AD). However, how such dysfunctions are established and whether they are functionally connected, remain mysterious. We have recently shown that ER subdomains that contact mitochondria or Mito-Associated Membranes (MAM) are hotspots for LD biogenesis, revealing the existence of a novel tripartite association between Mito, ER and nascent LD. We have found that two lipid transfer proteins ORP5 and ORP8 localize to MAM subdomains where LD originate and that their depletion decreases LD biogenesis in HeLa cells and human hepatocytes. We have also found that ORP5 interacts with seipin, a master regulator of LD biogenesis, and modulates its targeting to MAM-LD contacts. We hypothesize that MAM could play a key role in mediating Mito-LD crosstalk in health and in neurodegeneration.  We propose that the early cellular dysfunctions associated to AD involving Mito and LD could be all orchestrated by the MAM. We are currently investigating the function of ORP5/ORP8 and of their binding partners in lipid transfer and storage at MAM-LD contact sites as well as the morphology and remodelling of these contact sites in multiple cell lines including models of AD. Our studies aim to brings novel insights into the metabolic crosstalk between Mito, ER, and LD at membrane contact sites and their implication in neurodegeneration.

 

12 Apr 2024 14:30

Team 3: Guest seminar: Maxime Donadieu

Salle 52 (5e étage aile rouge - 5th floor red section)

 EAE models in marmoset for the assessment of cortical demyelination

Maxime Donadieu is a French visiting post-doctoral fellow. After a Bachelor in Biology with a major in Computational Neurosciences (2009-2012, Aix Marseille University), he decided to continue with a Master in Cellular Neurosciences (2013-2015, Aix Marseille University). He was recruited by Siemens S.A.S in 2015, in collaboration with the CRMBM lab (UMR 7339, Aix Marseille University), as an Engineer and also a PhD student. His PhD was focused on the development and the application of MR spectroscopy sequences on healthy subject and MS patients at high (3T) and ultra-high (7T) fields. He defended his PhD in December 2017 and moved in TNS lab in April 2018. The core of his project in TNS is to develop and use translational imaging (conventional, non-conventional MRI and PET scan) but also histology and immunochemistry on EAE marmoset model to understand and quantify the de/remyelination and neurodegenerative processes. The main objective of this project is to develop a remyelinating therapy relevant for MS patient.

19 Apr 2024 14:30

Team 11: Guest seminar : Valérie Delague

Salle 52 (5e étage aile rouge - 5th floor red section)

  • Point vulgarisation assuré par Gaelle Capraz sur "La poussière de cellules".

 

  • Valérie Delague is invited by Gaelle Capraz

 

  • Valerie Delague is a human geneticist and molecular biologist. While setting-up and leading the Molecular Genetics lab, during 7 years (1997-2004), at the Medical Genetics Unit (UGM) at Saint-Joseph University in Beirut, she obtained her PhD in Genetics in 2001. Since 2004, she is an Inserm Researcher in the MMG Research Unit, where she is focusing her research on Charcot-Marie-Tooth disease and other Inherited Peripheral Neuropathies, as an Inserm Research Director (DR2 Inserm) and leader of her own research group, “Genetics and physiopathology of Inherited Peripheral Neuropathies (IPN)”. She has a strong expertise in the genetics of this class of neuromuscular diseases with 62 publications, including the description of 3 new disease causing genes in the pathology. She is well-known for expertise in the field of recessive diseases in a context of consanguinity. She is also responsible of the Genomics and Bioinformatics Platform at the MMG.
  • She will present the platform's activities and technologies "Genomics and Bioinformatics Marseille"

Partners